FIG. 3.

Inhibition of wild-type and N1L1 phage infection with the different domains of g3p. (A) The inhibiting capacity of the different domains of g3p on the infection of F⁺TolA⁺ bacteria with VCSM13 wild-type phage was evaluated. In all experiments different amounts of phage were utilized (10³ to 10⁶ phage), together with a constant number (3 × 10⁷) of bacteria. The final concentration in each experiment of the g3p fragments were 8.0 μM N1, 3.4 μM N1L1, 2.6 μM N2, and 1.9 μM N1L1N2. The results are displayed as percent infection versus the value of wild-type infection, i.e., in the absence of the different g3p fragments. (B) The inhibition of N1L1 phage infection was evaluated in the same manner as the VCSM13 infection. The number of phage and bacteria utilized were the same as above. Furthermore, the final concentrations of the g3p fragments were the same in the two experiments, and the results are displayed as percent infection versus the value of N1L1 phage infection, i.e., in the absence of the different g3p fragments.