Fig. 2.

Allergic responses after intragastric challenge. (A) The responses of allergic symptoms: mice were evaluated 30 to 40 min after challenge and scored. The number of animals per group is showed in parentheses. (B) Images of representative reaction of negative control (PBS) and allergen (boiled extract) sensitized mice after challenge. Note the swelling of snout and mouth puffiness in the sensitized mouse but not in the PBS control mouse. Specific levels of IgE against (C) rTM, (D) raw shrimp extract and (E) boiled shrimp extract measured by ELISA. Relative mRNA expression of IL-4 (F IL-5), (G), IL-13 (H) and GATA-3 (I) of control and experimental groups (n = 8 per group). Data were normalized to HPRT-1. Data are expressed as mean ± SD. Data is considered statically significant when p < .05 on one-way ANOVA followed by Kruskal–Wallis test. * p < .05; ** p < .01; *** p < .001 and **** p < .0001