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. Author manuscript; available in PMC: 2024 Jun 23.
Published in final edited form as: Nat Plants. 2023 Aug 31;9(9):1398–1408. doi: 10.1038/s41477-023-01495-w

Figure 4. T-DNA concatenation increases gene editing efficiency.

Figure 4.

a. INDEL frequency of CRY2 PCR products amplified from DNA extracted from leaves of individual T1 plants transformed with either No RT or ONSEN RT constructs containing CRY2 sgRNA #1. b. Schematic representations of constructs used for gene targeting of ALS. SaCas9: Staphylococcus aureus Cas9. c. IPGT approach with ONSEN RT-induced T-DNA concatenation. d. Percentage of IM-resistant T2 plants from individual T1 parents transformed with No RT or ONSEN RT constructs. e. Summary of gene targeting events using No RT and ONSEN RT constructs. f. DNA-qPCR of ALS in ONSEN RT T1 plants (gray) in relation to percentage of IM-resistance for associated T2 plants (red).