Figure 2.

MED12 knockdown promotes enhanced cell proliferation and hyperactivated GLI3-dependent SHH signaling.

A) Proliferation assays of MED12 knockdown (shMED12) and control (shCT) cells. Data is mean ± SEM from 3 replicate assays. B) qRT-PCR of shCT and shMED12 cells using primers against the GLI3 target genes, GLI1 and CREB5. mRNA levels are normalized to GAPDH and expressed relative to their levels in shCT cells. Data is mean ± SEM from 3 replicate assays. (Unpaired two-tailed student t-test: ***P<0.0001, n.s: not significant).