Figure 3.

Bio-physical evaluation of interactions between RNA and ANP77 ligand. (A, B) Differential scanning calorimetry (DSC) spectra measured for (A) RNA oligomer and (B) for RNA-ligand complex in different pH conditions. (C-D) Cold-spray ionization time-of-flight mass spectrometry (CSI-TOF-MS) of G₂C₄ RNA (20 mM) in the (C) absence and (D) presence of ANP77 (50 mM). Asterisk (*) represents an ion corresponding to the 2– ion of dsG₂C₄ overlapping with the 1– ion of ssG₂C₄. The open triangle represents ion that could not be assigned. ssG₂C₄, dsG₂C₄, and tsG₂C₄ denote single-stranded, double-stranded, and tetra-stranded G₂C₄ RNA, respectively. (E, F) Circular dichroism spectra of G2C4 RNA (30 μM) measured in different pH (E) and temperatures (F).