Fig. 7.

FGF/ETV5 signaling regulates Piezo2 expression. a Prediction of ETV5 binding with Piezo2 promoter region. b ChIP-qPCR showed that ETV5 can bind to the genomic locus of Piezo2. P = 0.023 1, unpaired Student’s t-test, n = 3, and each point represents one biological replicate. c–f Expression of Etv5 in Fgfr1^fl/fl and Gli1-Cre^ER;Fgfr1^fl/fl mice at PN7.5. White arrows point to the expression of Etv5. White dashed lines outline HERS. g Relative expression of Etv5 in Negative control (NC) and Etv5 siRNA-treated apical mesenchymal cells with qPRC in vitro. P < 0.000 1, unpaired Student’s t-test, n = 3 and each point represents one biological replicate. h, i Expression of Etv5 in NC and Etv5 siRNA-treated group. j, k Expression of Piezo2 in NC and Etv5 siRNA-treated group. *P < 0.05, ****P < 0.000 1. Scale bars, 100 μm