Fig. 1.

Summary of the treatments used in this work. Arabidopsis Col-0 was treated with either isoxaben (ISX), which reduces cellulose biosynthesis, or Driselase (Dri), a combination of fungal enzymes that degrades cellulose, hemicelluloses, and pectins. For short-term treatments, 5-day-old roots were transferred to medium containing either 200 nM ISX or 0.05% Dri and left to grow for 4 h or 24 h. For constitutive treatment, Col-0 seeds were germinated and grown on medium containing 2 nM ISX or 0.03% Dri for 5 d prior to analysis. Mock-treated Col-0 seedlings were treated with an equal volume ethanol for 4 h. Images are maximum-projection images of PI-stained root tissue and are representative of six biological replicates. Scale bars are 50 µm.