Fig. 3.

Preincubation of T. vaginalis strain G3 with B7RC2-EVs increases adherence to host cells. (A) Representative fluorescence microscopy image of host cell adherence assay. T. vaginalis strain G3 was labeled with CFSE, then pretreated with BSA, G3-EVs, or B7RC2-EVs at 10 µg/mL for 30 min. Images depict CFSE-labeled T. vaginalis strain G3 (Green) and BPH-1 nuclei stained with Hoechst (Blue). (Scale bar, 100 µm.) (B) Quantification of parasite adherence to BPH-1. Data are depicted as fold change in adherence compared to BSA control. Bars, mean ± SD. N = 3 to 6 wells/experiment, four experiments total. Numbers above bars indicate P-values for one-way ANOVA, Dunnett’s multiple comparisons test compared to BSA control.