Fig. 4.

Preincubation of T. vaginalis strain G3 with B7RC2-EVs increases parasite aggregation. (A) Representative fluorescence microscopy image of parasite aggregation assay. T. vaginalis strain G3 was labeled with CFSE, then pretreated with BSA, G3-EVs, or B7RC2-EVs at 20 µg/mL for 30 min. Images depict CFSE-labeled T. vaginalis strain G3 (Green). (B) Quantification of parasite aggregate area. Bars, mean ± SD. N = 3 wells/experiment, three experiments total. (C) Quantification of parasite aggregate number. Bars, mean ± SD. N = 3 wells/experiment, three experiments total. (B and C) Numbers above bars indicate P-values for one-way ANOVA, Dunnett’s multiple comparisons test compared to BSA control.