Figure 6: Recombinase-expressing enhancer-AAVs.

A. Schematic representation of the vector design. B. Representative STPT images of coronal hemisections, showing labeling pattern for four individual enhancers expressing SYFP2 delivered to a wild-type mouse (left) and the same enhancers driving iCre(R297T), delivered to the Ai14 reporter mouse (right). A magnified view of VISp is shown alongside each hemisection. C. Heatmap showing the scoring of epifluorescence imaging data of the enhancers driving SYFP2 (rectangles) or a recombinase (circles); n = 39 pairs. D. Summary plot of the scoring data in (C), comparing brightness and specificity of the SYFP or recombinase expression. E. Specificity of Cre-dependent recombination in endothelial cells with the Ai2135m enhancer is reduced in iCre compared to the mutated version iCre(R297T) and is more sensitive to the viral dose (gc, genome copies). Scale bars in (B) and (E), 1.0 and 0.2 mm for full section and expanded view, respectively.