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Irf6 ablation in the neuroectoderm and neural crest changes Wnt1 expression domains within the neural folds. A. RNAscope in situ hybridization of transverse sections of Wnt1-Cre+;Irf6fl/fl cKO and littermate control E8 embryos. Rows represent 2 individuals of each genotype. Whereas Wnt1 expression (red) is localized to the caudal-dorsal neural folds in the control embryos, Wnt1 expression in Wnt1-Cre+;Irf6fl/fl cKO embryos is displaced laterally (arrows). Blue is dapi. Scale: 100 μM
