FIG. 8.

Growth of recombinant HIV-1 containing 5′ LTNP 5 sequences. LTNP 5 and NL 4-3 sequences from bp 297 to 4402 (5′) and NL 4-3 sequences from bp 4402 to 9607 (3′) were amplified separately. An XmaI (CCCGGG) site was introduced at the junction of the PCR fragments (bp 4402) as described in Materials and Methods. XmaI-digested 5′ LTNP 5 and 5′ NL 4-3 fragments were cotransfected with 3′ NL 4-3 fragments into permissive CEMx174 cells. Supernatants were collected and assayed for p27 antigen concentration.