Table 2. Thermocycling conditions used to identified Batrachochytrium dendrobatidis presence or absence using polymerase chain reaction (initial - PCR).

STEP	Temperature	Time	Cycles
Initial denaturation	93 °C	10 min	X 1
Denaturation	93 °C	45 s	X 30
Annealing	60 °C	45 s
Extension	72 °C	1 min
Final extension	72 °C	10 min	X 1
	4 °C	¥