TABLE 1.
Primers used in preparation of KUN replicon vectors and constructs
| Primer | Sequence (5′→3′)a | Restriction site used |
|---|---|---|
| Ub_F | ggttctagatgcatacgcgttaCAGATATTCGTGAAGACT | XbaI |
| Ub_R | ggtactagtacgtaaggcgcGCCACCACGGAGACG | SpeI |
| NS5dGDD_F | CTG GTT AAC TGT GTG GTA AAG CCC TT | |
| 3′UTRHDV | GAGAACACAGGATCTGGGTCGGCATGGCATCT | |
| SV40Pa_R | ggcctcgaGCAATTGTTGTTGTTAACTT | XhoI |
| CMV_F | gcgcttaaGACATTGATTATTGACTAGTTA | |
| CMV5′UTR | CGTTTAGTGAACCGAGTAGTTCGCCTGTGTGA | |
| FMDV2A_R | gtgacgcgtcggccGGGCCCTGGGTTGGA | EagI |
| PacPst_F | cgccctgcagccACCGAGTACAAGCCCA | PstI |
| 2AMluNsi_R | ctggatgcatacgcgtcGGGCCCTGGGTTG | NsiI |
| NsiIRES_F | ggtatgcatagCGGGATCAATT | NsiI |
| dNS1H3_R | ccgaagcttgTCCAGTATCAGCATGCA | SphI |
| NsiLacZ_F | ccggatgcatggGTCGTCTTACAGCGTCGT | NsiI |
| NsiLacZ_R | cgcgatgcatgTTTTTGACACCAGACTAACT | NsiI |
a
Nucleotides in capital letters represent the authentic KUN or cloned heterologous sequences (defined in the text), nucleotides in lowercase letters show artificial sequences, and nucleotides in boldface show introduced restriction sites used in cloning.