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Sera from BDV strain V-infected rats recognizes recombinant 190-kDa L. Metabolically labeled lysates obtained from pTM-L1-transfected BSR-T7 cells (lanes 1 to 4) or mock (vector)-transfected BSR-T7 cells (lanes 5 to 8) were immunoprecipitated using anti-L1 antisera (lanes 1 and 5), rat sera 2 months (lanes 2 and 6) or 4 months (lanes 3 and 7) post-BDV infection, or noninfected rat sera (lanes 4 and 8). Immunoprecipitation analysis was done by SDS-7.5% PAGE.
