Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Homo sapiens) | PKA-Cα | PKA-C | Uniprot ID: P17612 |
|
| Strain, strain background (Escherichia coli) | BL21(DE3) pLyss | Agilent | Cat. #200132 | Chemically competent cells |
| Sequence-based reagent | PKA-CF100A | This study | PCR primer (Forward) |
tattctgcaagcggtgaacgccccgtttctggttaagctg |
| Sequence-based reagent | PKΙα 5-24 | Synthetic peptide | PKI5-24 | Chemically synthesized |
| Sequence-based reagent | Kemptide | Synthetic peptide | Kemptide | LRRASLG |
| Commercial assay or kit | QuikChange Lightning Multi Mutagenesis Kit | Agilent genomics | Cat #210519 | Commercial mutagenesis kit |
| Recombinant DNA reagent | PKA-CF100A | This study | PKA-CF100A | Single Ala mutant of PKA-C |
| Chemical compound, drug | AMP-PNP or ATPγN | Roche Applied Science | CAS 25612-73-1 | ATP analogous |
| Chemical compound, drug | ADP | Sigma-Aldrich | CAS 20398-34-9 | Nucleotide |
| Software, algorithm | TopSpin 4.1 | Bruker Inc | https://www.bruker.com/ | |
| Software, algorithm | NMRFAM-Sparky | NMRFAM | https://nmrfam.wisc.edu/nmrfam-sparky-distribution/ | |
| Software, algorithm | NMRPipe | Delaglio F., NIH | https://www.ibbr.umd.edu/nmrpipe/install.html | |
| Software, algorithm | POKY | Lee W. | https://sites.google.com/view/pokynmr | |
| Software, algorithm | COordiNated ChemIcal Shift bEhavior (CONCISE) | Veglia G. |
https://conservancy.umn.edu/handle/11299/217206
https://conservancy.umn.edu/handle/11299/227294 |
Matlab script |
| Software, algorithm | CHEmical Shift Covariance Analysis (CHESCA) | Melacini G. | https://academic.oup.com/bioinformatics/article/37/8/1176/5905475?login=true | NMRFAM-Sparky &POKY tools |
| Software, algorithm | PyMol | Schrödinger, LLC | https://pymol.org | |
| Software, algorithm | MatLab2022b | MathWorks | https://www.mathworks.com/products/matlab.html | |
| Software, algorithm | GraphPad Prism 9 | GraphPad Software Inc | https://www.graphpad.com/ | |
| Software, algorithm | GROMACS 4.6 | Hess B et al. | http://www.gromacs.org/ | |
| Software, algorithm | CHARMM36a1 | Best et al., 2012 | https://doi.org/10.1021/ct300400x | |
| Software, algorithm | PLUMED 2.1.1 | Bonomi et al., 2009 | https://www.plumed.org/doc-v2.5/user-doc/html/_c_h_a_n_g_e_s-2-1.html | |
| Software, algorithm | ALMOST 2.1 | Kohlhoff et al., 2009. | https://svn://svn.code.sf.net/p/almost/code/almost-code | |
| Software, algorithm | METAGUI | Biarnés et al., 2012 | https://www.sciencedirect.com/science/article/pii/S0010465511003079 | |
| Software, algorithm | MDTraj | McGibbon et al., 2015 | https://www.sciencedirect.com/science/article/pii/S0006349515008267 | |
| Software, algorithm | SPARTA+ | Shen and Bax, 2010 | https://spin.niddk.bov/bax/software/SPARTA+/ | |
| Software, algorithm | MSMbuilder | Harrigan et al., 2017; | https://msmbuilder.org/ | |
| Software, algorithm | Mutinf | McClendon et al., 2012 | https://simtk.org/projects/mutinf/ |