Author response image 1. Comparison of transcriptome data in different KO and KD experiments.

a) Correlations between biological replicates of Dux/Obox4 DKO pseudo-bulk scRNA-seq (left panel) and ASO mediated Dux/Obox4 DKD RNA-seq (right panel). DKO samples bear higher inter-sample variance than DKD samples. 2C-genes are highlighted, inter-sample transcriptome correlations are measured by coefficient of determination (R²). b) Correlations of gene expression changes between Dux/Obox4 DKO and DKD RNA-seq. RNA-seq of DKD provides higher statistic power at discovering down-regulated 2C genes. 2C-genes are highlighted. c) Correlations of 2C-gene expression changes between published Dux KO RNA-seq and our ASO-mediated Dux KD RNA-seq. Our KD transcriptome shows higher agreement with the two KOs produced by Guo et al. and Chen et al. than between them each other.