Fig. 4. Long-term comparison of storage performance in E-Sol 5 at +4 °C and −5 °C after 3, 21, 22, and 23 weeks of storage.

Samples were assessed for their supernatant colors where redness is proportional to hemolysis. At week 21 and later time points, the hemolysis for the samples stored at +4 °C was significantly higher than the hemolysis for the samples stored at −5 °C. Black dashed line represents the mean day 1 level following 3x washing, overnight storage at +4 °C, and final 1x washing in E-Sol 5. Data represent mean ± standard deviation from 2 biological replicates (N = 2) and 3 technical replicates (n = 3) for each biological replicate. Each biological replicate was from a pool of 3 donor samples. A two-way analysis of variance (ANOVA) followed by Tukey’s post hoc test was performed to evaluate significant differences between conditions: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Comparisons were shown across the three temperatures at each time point. See Supplementary Fig. 7 for comparisons across the time points at each temperature.