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. 2024 Jun 24;14:14471. doi: 10.1038/s41598-024-65173-3

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression levels of ACKR1 on venular endothelium of omentum and cremaster. Confocal microscopic images of venules in the cremaster muscle or the omentum (as indicated on the left) stained for PECAM-1 (red) and ACKR1 (magenta); bar = 20 µm. (b) Quantification of staining intensity for ACKR1 normalized for the staining intensity of PECAM-1 was determined for total ACKR1 (left graph), for junctional ACKR1 (middle graph) and for surface ACKR1 outside of junction areas (right graph). A total of 70 vessel segments were evaluated (n = 18 VEC-WT cremaster, n = 24 VEC-αC cremaster, n = 10 VEC-WT omentum, n = 18 VEC-αC omentum) and presented as mean ± SEM. All vessel segments had diameter ranges from 60 to 85 μm. ****P < 0.0001; two-way ANOVA.