Extended Data Fig. 1. Alloreactive T cells alter intestinal and circulating BAs and host metabolism.

Lethally irradiated 6–8 week old female BALBc mice were transplanted with 10 × 10⁶ BM cells alone (BM) or together with 1 × 10⁶ T cells (BM+T). (a-c) BAs were quantified on day 7 post-transplant by liquid chromatography-mass spectrometry (LC-MS) in the cecal contents and plasma. (a) Ratio of microbe- to host-derived BAs (b) Ratio of unconjugated to glycine- and taurine-conjugated BA. (c). Estimated cecal levels of the T cell modulatory BAs 3-oxoLCA and isoLCA (below limit of detection). Weight loss (a), clinical GVHD scores (b) and cumulative food intake per mouse at day 7 post-transplant (c). Plasma levels of AST (d), ALT (e), albumin (f), cholesterol (h), and triglycerides (g). Data combined from two independent experiments (n=10). Data shown as mean ± S.D and statistical significance determined by two-tailed Mann-Whitney test.