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. 2024 Jun 10;15:1388272. doi: 10.3389/fimmu.2024.1388272

Figure 3.

Figure 3

pHx induces neutrophil accumulation and increases bacterial abundance in the residual liver. (A) Flow cytometric analysis with staining of different populations of innate immune cells in healthy liver tissue (neutrophils: CD45+/CD11b+/Ly6G+/CD64-, monocytes: CD45+/CD11b+/Ly6G-/CD64+) for PO (n=21), SHAM (n=8) and pHx (n=19) group, (B) Time course of infiltration of the liver with innate immune cells before POD5 (range POD3–5) and after POD5 (range POD7–14) for PO (n=21), SHAM<POD5 (n=3), SHAM>POD5 (n=6), pHx<POD5 (n=5) and pHx>POD5 (n=14) group; (C) Concentration (ng/µl) of bacterial DNA in healthy liver measured with qPCR for Ctrl (n=5), SHAM (n=5) and pHx (n=7) group; (D) FACS analysis of different populations of adaptive immune cells in healthy liver tissue following pHx (NK cells: CD45+/CD3-/NK1.1+, T cells: CD45+/CD3+/NK1.1-, B cells: CD45+/CD3-/B220+) for PO (n=20), SHAM (n=14) and pHx (n=25) group; (E) Representative immunohistochemistry of the distribution of CD4+ and CD8+ T cells in healthy liver and accumulation in premetastatic lesions (top) with absence in macroscopic metastasis (bottom). Red arrows point to the cells of interest. (Scale bars = 100 µm, bar plots represent mean ± SEM). n.s. non significant.