Figure 4.

Partial hepatectomy is associated with upregulation of gene sets associated with metastasis in the liver. (A) Volcano plot of differently expressed genes (DEGs) in RNA sequencing from healthy liver tissue of 4 pHx and 4 PO samples. The 10 DEGs with the highest or lowest log2 fold change were labeled; (B) Heatmap from all DEGs with a base count mean of > 10 visualized by Z-scores; (C) Overview of 5 most enriched and 2 most depleted hallmark terms in gene set analysis (GSEA) following pHx; (D) Cnet plot visualizing all DEGs related to hallmarks E2F targets and G2M checkpoint and their overlap; (E-G) GSEA for hallmarks angiogenesis (E), hypoxia (F) and inflammatory response (G) and heatmap of the respective 10 leading edge genes (right) visualized by Z-scores; (H) Cnet plot of DEGs related to hallmarks angiogenesis, hypoxia, and inflammatory response.