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. 2024 May 15;19(6):1243–1249. doi: 10.1021/acschembio.4c00151

Figure 2.

Figure 2

NAD cap of U1 snRNA destabilizes the complex with HIV-1 pre-mRNA. (A) Scheme of the complex formed by complementary regions of HIV-1 pre-mRNA with either TMG-U1 (top) or NAD-U1 (bottom). Nucleobase symbols in red mark positions mutated to meet the requirements of T7 RNA polymerase. (B) Duplex stability of HIV-1 pre-mRNA with TMG-U1 (dark gray) or NAD-U1 (blue) measured using a light cycler device (measured in triplicate in a series of nine measurements of each, t-test).