|
| Bacterial and virus strains |
|
| NEB 5-alpha Competent E. coli |
New England Biolabs |
N/A |
| S17–1 λpir
|
Simon et al. (1983)70
|
N/A |
| SM10 λpir
|
Simon etal. (1983)70
|
N/A |
| MPAO1 |
Jacobs et al. (2003)68
|
N/A |
| MPAO1 ΔxdhA
|
This study |
N/A |
| MPAO1 ΔaptΔhgptΔxpt
|
This study |
N/A |
| MPAO1 ΔaptΔhgptΔxpt attB::hgpt
|
This study |
N/A |
| MPAO1 ΔrelAΔspoT
|
This study |
N/A |
| MPAO1 ΔrelAΔspoTΔaptΔhgpt
|
This study |
N/A |
| MPAO1 ΔrelAΔspoTΔaptΔhgptΔxpt
|
This study |
N/A |
| MPAO1 ΔrelAΔspoTΔaptΔhgptΔxpt attB::hgpt
|
This study |
N/A |
| MPAO1 ΔguaB
|
This study |
N/A |
| PAK |
Takeya and Amako (1966)29
|
N/A |
| PA14 |
Schroth et al. (2018)30
|
N/A |
|
| Chemicals, peptides, and recombinant proteins |
|
| Acetic Acid |
Fisher Scientific |
A38–212 |
| Acid Casein Peptone |
Fisher Bioreagents |
BP1424–100 |
| Adenine |
Alfa Aesar |
A14906 |
| Adenosine |
TCI |
A0152 |
| Agar |
Fisher Bioreagents |
BP1423–500 |
| Ammonium Chloride |
Fisher Chemical |
A661–500 |
| Arginine |
Dot Scientific |
DSA50400–100 |
| Breathe-Easy Film |
USA Scientific |
9123–6100 |
| Carbenicillin |
Sigma-Aldrich |
C9231–1G |
| Costar 24-well Clear TC-treated Multiple Well Plates |
Corning |
3526 |
| Dextrose (Glucose) |
Sigma-Aldrich |
D9434–500G |
| Gentamicin |
TCI |
G0383 |
| Gibson Assembly Master Mix |
New England BioLabs |
M5510AA |
| Glycerol |
Sigma-Aldrich |
G5516–1L |
| Guanosine |
TCI |
G0171 |
| HinDIII-HF |
New England BioLabs |
R3104S |
| Hypoxanthine |
Thermo Scientific |
122010050 |
| Inosine |
TCI |
I0037 |
| Irgasan |
Sigma-Aldrich |
72779–5G-F |
| Luria-Bertani Powder |
Fisher Bioreagents |
BP1427–2 |
| Magnesium Sulfate Heptahydrate |
Sigma-Aldrich |
BP213–1 |
| Nunc MicroWell 96-Well Microplates |
Thermo Scientific |
167008 |
| Nunc MicroWell 96-Well Optical-Bottom Plates with Black Polymer Base |
Thermo Scientific |
165305 |
| Phusion Green Hot Start II High-Fidelity PCR Master Mix |
New England BioLabs |
F566L |
| Potassium Phosphate Monobasic |
Sigma-Aldrich |
795488–500G |
| Safranin O |
Alfa Aesar |
B21674.18 |
| Sodium Chloride |
Sigma-Aldrich |
S9888–10KG |
| Sodium Phosphate Dibasic |
Sigma-Aldrich |
S7907–500G |
| Sucrose |
Sigma-Aldrich |
S0389–1KG |
| Tryptone |
Fisher Bioreagents |
BP1421–2 |
| Xanthosine |
TCI |
X0008 |
| Yeast Extract |
Fisher Bioreagents |
BP1422–500 |
|
| Oligonucleotides |
|
| See Table S1
|
|
| Recombinant DNA |
|
| pCdrA::GFP(ASV) |
Rybtke et al. (2012)28
|
N/A |
| pEXG2 |
Rietsch et al. (2005)69
|
N/A |
| pEXG2-xdhA |
This study |
N/A |
| pEXG2-APT |
This study |
N/A |
| pEXG2-HGPT |
This study |
N/A |
| pEXG2-XPT |
This study |
N/A |
| pEXG2-guaB |
This study |
N/A |
| pEXG2-relA |
This study |
N/A |
| pEXG2-spoT |
This study |
N/A |
| pminiCTX-1 (GmR derivative) |
Hoang et al. (2000)71
|
N/A |
| pminiCTX-1-HGPT |
This study |
N/A |
| pFLP2 |
Hoang et al. (1998)73
|
N/A |
|
| Software and algorithms |
|
| GraphPad Prism v10.2.2 |
GraphPad |
www.graphpad.com
|
| ImageJ |
Available from NIH |
https://imagej.net
|
