Fig. 2.

Anti-ac-tauK174 antibody Clone 1 rescues neurobehavioral impairment in P301S (PS19) mice. (A) Experimental timeline. At 6 months of age, male and female P301S mice were peripherally (IP) injected with Clone 1 (25 mg/kg) or vehicle (PBS). Non-transgenic (WT) littermates were injected with PBS and are included as controls. All mice received a single injection weekly for a period of 15 weeks. Behavioral analysis was performed during the last 4 weeks of treatment. Mice were sacrificed at 10 mo following the last injection. (B) Percentage of weight loss at the endpoint (week 15) among three groups of animals, normalized to the start point (week 1). ***p < 0.001, *p < 0.05 by one-way ANOVA, Sidak’s multiple comparison test. (C, D) Motor coordination impairment was measured by hindlimb extension test. Each mouse was suspended by its tail for 10 s and its hind-limb posture was scored as 1, 0.5, or 0 (C). The average score for each group is presented (D). ***p<0.001, **p < 0.01 by one-way ANOVA, Sidak’s multiple comparison test. (E, F) Spatial learning and memory were measured by Morris water maze (MWM). (E) Learning curve during the training phase. ***p < 0.001, *p < 0.05 by two-way ANOVA, Tukey’s multiple comparison test. (F) Probe trial 72 hr post-training performed on Day 8. **p < 0.001, *p < 0.05 by paired t-test. n = 14 per group