Fig. 1.

Profiling of B cell subsets. A Gating strategy to obtain the frequency of plasmablast (PB) in each sample using traditional flow cytometry. B Plasmablast frequency correlation with age in patients with acute transverse myelitis using traditional flow cytometry. C Plasmablast frequency correlation with age in healthy controls using traditional flow cytometry. D–G Plasmablast subset frequencies in pediatric acute transverse myelitis and healthy controls using CyTOF data. H, I TSNE plots of CyTOF data identifying 20 distinct B cell subsets in the cohort