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. 2024 Jun 24;21:161. doi: 10.1186/s12974-024-03127-2

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Plasmablast rhAbs bind neurons and astrocytes in mouse spinal cord. A, B Representative images of rhAbs binding neurons and astrocytes in the lumbar spinal cord of WT (A) and EAE (B) mice. Green: IgG staining. Red: GFAP. Blue: MAP2. In the merge panels, yellow indicates co-stain of GFAP with the rhAb. The MFI of astrocytic staining is indicated in parentheses. Scale bar: 20 μm. C Pie charts summarizing the number of rhAbs binding astrocytes in WT and EAE spinal cord tissue. D Scatter plots of the mean fluorescent intensity of the 11 astrocyte-binding rhAbs in EAE spinal cord tissue where the rhAbs are binned according to cohort (ALL, HC or ATM) and somatic hypermutation (SHM) accumulation status of the antibody heavy chain where “SHM−” indicates there is no SHM in the rhAb heavy chain and “SHM + ” indicates there is SHM in the rhAb heavy chain. Horizontal dashed line indicates the threshold of positivity which is set at 366.3. The average MFI of each cohort is provided below the x-axis