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. 2000 May;74(10):4541–4548. doi: 10.1128/jvi.74.10.4541-4548.2000

FIG. 3.

FIG. 3

Virus overlay assay (bottom panel) of alanine replacement mutants of MpB GroEL(1-408) to identify individual amino acids involved in PLRV binding. Lane 1, wild-type MpB GroEL isolated from M. persicae; lane 2, recombinant MpB GroEL. All other lanes contain wild-type MpB GroEL(1-408) and point mutants of MpB GroEL(1-408) as indicated. The positions of full-length MpB GroEL, MpB GroEL(1-408), and alanine replacement mutants of MpB GroEL(1-408) are indicated by arrows. (Top panel) Amido black-stained blot.