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Virus overlay assay (bottom panel) of alanine replacement mutants of MpB GroEL(122-548) to identify individual amino acids involved in PLRV binding. Lanes contain wild-type MpB GroEL(122-548) and point mutants of MpB GroEL(122-548). The positions of MpB GroEL(122-548), and alanine replacement mutants of MpBGroEL(122-548) are indicated by arrowheads. (Top panel) Amido black-stained blot.
