TABLE 1.

Oligonucleotides used for the construction of MpB GroEL deletion mutants

Oligonucleotide	Sequence (5′→3′)a	Corresponding positionsb
F1	ccggatccATGGCCGCTAAAGATGTA	1–6
F2	ggatccatgAAAGCTGTTATTAGTGCTG	122–127
F6	ggtgaagcttAACTATGGTTATAATGCAGC	475–480
F7	ccggatccGGTAATGAAGCCCGAATT	9–14
F8	ccggatccGGAGTTAATGTATTAGCAG	19–24
F9	gaggatccGTAACTTTAGGTCCAAAAG	29–34
F10	ttccaagcttTTATCTGTACCATGTTCAG	134–139
R1	acggatccTTACATCATTCCaCCC	545–548
R2	gaattcttaACCTTTTCCATCTTTTACG	470–474
R3	caataagcttTTCAACAGCTGCACCAGT	404–408
R4	gaattcttaTTCAACAGCTGCACGAG	404–408
R7	caataagcttGATTTCACGAGCTACTGA	53–57
R8	gaattcttaATTGGAAACAATTTGACGTAA	452–457
R9	gaattcttaAGATGTTTTTCCAGCCACTC	422–427

^aUppercase letters indicate Buchnera groEL sequences, and lowercase letters indicate sequences which are not part of the gene. Restriction sites (EcoRI, BamHI, and HindIII) are boldfaced, start codons are double underlined, and termination codons are single underlined. 

^bNumbering refers to the corresponding positions of the amino acid residues of MpB GroEL.