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Pulse-chase analysis of Us9. PK15 cells were infected with either PRV Be (wild type) or PRV 162 (del 46–55) at an MOI of 10. The cells were pulse-labeled with 125 μCi of [35S]methionine-cysteine for 7 min, rinsed with PBS, and chased for the times indicated. Cellular lysates were immunoprecipitated with a Us9 polyvalent antiserum and fractionated on a 12.5% polyacrylamide gel. Molecular mass markers (kilodaltons) are indicated on the left.
