FIG. 7.
Localization of Us9 alanine scanning mutant viruses in the brain. Male Sprague-Dawley rats were subjected to intravitreous injection with approximately 5 × 105 PFU of PRV 170 (Y49–50A S51A S53A), PRV 171 (E52A D54A N55A E56A), PRV 172 (Y49–50A), or PRV 173 (S51A S53A). The localization of viral antigen in 35-μm-thick coronal sections was detected with an antigen against whole-virus particles (Rb133). Each vertical set of three panels shows sections from one animal, and the time to which the animal survived after infection is indicated. Representative samples are shown for each virus. Images of brain tissues from animals infected with PRV Be (1.2 × 106 PFU) are included for comparison (7).