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. 2000 May;74(10):4549–4561. doi: 10.1128/jvi.74.10.4549-4561.2000

TABLE 3.

Summary of intravitreal injections of Us9 alanine scanning mutant viruses

Animal no. Virus Genotype Time to death (h) Spreada Symptomb Plaque sizec Endocytosisd
99-AB17 PRV Bee Wild type 70.3 +++ Wild type +++ +++
99-AB18 PRV Be Wild type 63.6 +++ Wild type +++ +++
99-AB10 PRV 162 del 46–55 79.5 − Attenuated +++ −f
99-AB11 PRV 162 del 46–55 75.8 − Attenuated +++ −
99-AB12 PRV 162 del 46–55 73.0 − Attenuated +++ −
99-AB15 PRV 166 L30–31A 74.8 +++ Wild type +++ ++f
99-AB16 PRV 166 L30–31A 64.4 +++ Wild type +++ ++
99-AB25 PRV 170 Y49–50A S51A S53A 86.6 − Attenuated +++g −
99-AB26 PRV 170 Y49–50A S51A S53A 68.7 − Attenuated +++ −
99-AB33 PRV 170 Y49–50A S51A S53A 98.3 − Attenuated +++ −
99-AB34 PRV 170 Y49–50A S51A S53A 96.8 − Attenuated
99-AB27 PRV 171 E52A D54A N55A E56A 113.6 ++ Attenuated +++g +
99-AB28 PRV 171 E52A D54A N55A E56A 86.8 − Attenuated +++ +
99-AB35 PRV 171 E52A D54A N55A E56A 77.9 − Attenuated +++ +
99-AB36 PRV 171 E52A D54A N55A E56A 68.2 − Attenuated +++ +
99-AB29 PRV 172 Y49–50A 85.3 − Attenuated +++g ++
99-AB30 PRV 172 Y49–50A 73.5 − Attenuated +++ ++
99-AB37 PRV 172 Y49–50A 87.3 − Attenuated +++ ++
99-AB38 PRV 172 Y49–50A 78.3 − Attenuated
99-AB31 PRV 173 S51A S53A 70.3 − Attenuated +++g +
99-AB32 PRV 173 S51A S53A 84.0 + Attenuated +++ +
99-AB39 PRV 173 S51A S53A 71.5 − Attenuated +++ +
99-AB40 PRV 173 S51A S53A 74.0 − Attenuated +++ +
a

Relative amount of PRV viral antigen found in the SC and DGN compared to that found after PRV Be infection. +++, amount found after PRV Be infection. 

b

Wild-type versus attenuated symptoms have been described previously (7). 

c

Relative sizes of plaques measured 48 h after infection compared to sizes of plaques formed by PRV Be (+++). 

d

Endocytosis was examined by antibody uptake assays and indirect immunofluorescence internalization assays (data not shown) on PK15 cells stably expressing the various mutant Us9-EGFP fusion proteins. The relative amount of the mutant Us9–EGFP fusion proteins internalized is compared to that of wild-type Us9–EGFP (+++). 

e

The data for animals infected with PRV Be have been reported previously and are included for comparison (7). 

f

The endocytosis phenotypes for PRV 162 and PRV 166 have been described previously (8). 

g

Data not shown.