Expression landscape during embryogenesis in allotetraploid common carp. (a) Sampling stages during embryogenesis. S1, morula; S2, blastula; S3, gastrula; S4, neurula; S5, optic vesicle; S6, tail bud; S7, muscle contraction; S8, hatched larvae. Microscope images were modified according to Jin et al. [110]. (b) Expression patterns of DEGs during embryogenesis. DEGs were identified by pairwise comparison between two adjacent stages at 24 °C and termed stage-related DEGs or SDEGs. The heatmap shows the overall expression of all the SDEGs, indicating the similarity between S6 and S7. (c) Functional enrichment of genes in seven clusters in Fig. 3b. (d) Percentage of single or paired homoeologs in each of the seven clusters. Paired means that the two homoeologous genes in the two subgenomes existed in the same cluster.