FIG. 1.

Construction of pCVB3-PL2-Ad2L1. (a) The construction of the recombinant plasmid using a CVB3/0-derived subclone, pBSPL2, is described in Materials and Methods. The CVB3 ORF is indicated by open bars, the Ad2 hexon L1 loop insert is indicated by a solid box, and the polylinker is indicated by a shaded box. Regions encoding 2Apro recognition sites are indicated by black arrows. NTR, nontranslated region. PolyA, poly(A) tract located at the 3′ end of the CVB3 genome. (b) The nucleotide sequence of the polylinker with the flanking dissimilar duplicated region that encodes the modified 2Apro cleavage site (shaded bar) is given. Nucleotide numbering is based on the CVB3/0 genome found at GenBank accession no. M88483.