FIG. 2.
Replication of CVB3-PL2-Ad2L1 in cell culture. (a) Single-step growth curves of CVB3-PL2-Ad2L1 and CVB3/0 in HeLa cells were obtained as described in the text. Cultures were harvested by freezing at the indicated times postinoculation. Virus titers were determined on HeLa cell monolayers and are expressed as the logarithm of TCID50 per milliliter with each data point representing the mean titer and standard deviation of triplicate independent experiments. (b) Yields of infectious virus in MFHF, HCAEC, and COS-1 cultures 24 h after inoculation with CVB3-PL2-Ad2L1 or CVB3/0.