FIG. 3.

Western blot analysis of viral proteins in CVB3-PL2-Ad2L1-inoculated HeLa cells. Proteins were harvested 5 and 7 h postinoculation from HeLa cell monolayers inoculated with either CVB3-PL2-Ad2L1 or CVB3/0, separated by SDS-polyacrylamide gel electrophoresis, transferred to nitrocellulose, and probed with an anti-CVB3 antibody. Anti-CVB3 primary antibody was detected with a horseradish peroxidase-linked second antibody, and the signal was developed using an enhanced chemiluminescence system (ECL⁺; Amersham). Molecular masses at which the CVB3 capsid protein (CVB3-1D) and the chimeric protein (CVB3-1D/Ad2-L1) migrate are indicated.