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. Author manuscript; available in PMC: 2024 Jun 25.
Published in final edited form as: Mucosal Immunol. 2020 Jun 23;14(1):135–143. doi: 10.1038/s41385-020-0306-6

Fig. 1. Eliciting antigen is restricted to the SI during the onset of food-induced anaphylactic symptoms.

Fig. 1.

(A) Experimental regimen, (B) Localization of OVA-fluorescence in the GI tract segments of naïve or food allergic WT and iIL-9Tg mice following anti-IgE treatment. OVA-sensitized BALB/c mice were repeatedly challenged with OVA and on the 7th challenge received 5 x 105 FITC-labelled FluoSpheres™ Polystyrene Microspheres and localization of FluoSpheres in the GI segments were examined by fluorescence within 30 min. Naïve mice and iIL-9Tg mice challenged with either anti-IgE (20μg / 200μl i.v.) or vehicle received oral gavage of 5 x 105 FITC-labelled FluoSpheres™ Polystyrene Microspheres and localization of FluoSpheres in the GI segments were examined within 30 min. Data are represented as the mean fluorescence signal detected in luminal contents (per mg) of the respective GI segments from n = 5 mice.