FIG. 2.

Correction of AP mutations with different AAV targeting vectors. Standard AP gene correction assays were performed on polyclonal fibroblast populations containing each of the indicated AP target mutations with AAV-3′AP-Bam, AAV-5′AP-Psh, AAV-5′AP-Bss, and AAV-3′AP-(B/A). Each value represents the average from at least two independent experiments. (A) Correction rates were plotted for each AAV targeting vector, with the total length of homology each AAV vector shared with the target locus indicated. The data are arranged with increasing total homology length from left to right. (B) The data in panel A are plotted relative to the short end homology extension for each correction assay. The short end homology extension is the distance of the corrected mutation from the end of the short side flanking homology.