FIG. 6.

Effects of PNA_PR2 on gag, pol, and env gene expression and virion production in COS-7 cells transfected with an infectious HIV-1 molecular clone, pNL4-3. Shown are results of Western blot analyses with anti-p24^Gag antibody and anti-p17^Gag antibody (A), antiprotease antibody and anti-p66^RT antibody (B), and anti-gp120 antibody (C) of pNL4-3-transfected COS-7 cells cultured with no drug (lanes 1), 100 μM PNA_PR2 (lanes 2), 1 μM saquinavir (also a protease inhibitor) (lanes 3), or 3 μM indinavir (lanes 4). The cell lysate of mock-transfected COS-7 cells is shown in lanes 5. Viral lysate from pelleted virion particles (vp) is included in the Western blot analyses for the pol gene products as a reference. The levels of p24 antigen in supernatant and the infectious titers determined by MAGI assay are shown at the bottom. Ab, antibody; Ag, antigen.