FIG. 2.

Effect of Vpr expression on IL-8 promoter activity in A549 cells. A549 cells transfected with 2 μg of full-length IL-8 promoter-CAT construct (pIL-8CAT-1481) and increasing amounts of Vpr expression plasmid pSVCMVER or A549 cells transfected with pIL-8CAT-1481 and treated with IL-1α (4 ng/ml) were assayed for IL-8 promoter activity by CAT ELISA. To eliminate variable transfection efficiencies due to differing amounts of plasmid DNA, final amounts of plasmid DNA for each transfection were adjusted to 4 μg using pSVCMV.