Figure 6.

Influence of antibody format on neutralization efficacy. MRU44–4-A1 and MRU44–4-A10 were tested in IgG, scFv-Fc and Fab format in neutralization assay. 210 nM of IgG or scFv-Fc antibody and 420 nM of Fab antibodies were prepared and diluted down to 0.07 nM and 0.13 nM respectively, and incubated with 4.2 nM α-LTX. Used Fab were stoichiometrically corrected to F(ab)₂ molecules, to compare same quantity of binding moieties. Mean value of mock intoxication, treating the cells without toxin is marked in long dashed lines, mean value of treating cells with α-LTX without adding antibodies is marked in short dashed lines. An unrelated scFv-Fc was included as control. IC₅₀ values of different MRU44–4-A1 antibody formats were determined by using Origin Hill1 non-linear fit (see also Table 2 ). Antibodies were tested in duplicates and experiment repeated as a biological replicate with a fresh batch of PC-12 cells. One of the two repetitions is shown as a representative result. Error bars represent the standard deviation of two measurements.