Figure 7.
Size exclusion chromatography (SEC) and neutralization efficacy after lyophilization. (A) SEC measurement of antibodies selected for lyophilization. Antibodies were reconstituted in ddH2O and measured in SEC following lyophilization. (B) Neutralization assay of antibodies selected for lyophilization. Antibodies were stored at 4°C and -80°C, respectively. Lyophilized antibodies were reconstituted with ddH2O and compared to stored antibody samples. Antibodies were prepared with 210 nM and titrated to 0.07 nM and incubated with 4.2 nM α-LTX. Afterwards cells were intoxicated with toxin:antibody preincubation mix as described. Assay development was done according to screening of lead IgG. Error bars represent the standard deviation of two measurements.