Table 1.

Unique scFv (by sequence) that bound α-LTX per number of positive hits per number of clones analyzed in α-LTX ELISA.

Library	α-LTX coated MTP	Captured α-LTX	α-LTX in Solution
HAL9 (kappa)	5/23/184	–	0/0/92
HAL10 (lambda)	0/0/184	–	0/0/92
Library Mix (HAL9+HAL10)	69/213/1336	1/3/276	–

MTP, multitier plates, Captured: biotinylated α-LTX was immobilized on MTP coated with Streptavidin, Panning in solution was done by incubating the libraries with biotinylated α-LTX in solution, to be captured after 60 minutes on MTP coated with Streptavidin.

In total, 75 unique antibodies binding L. tredecimguttatus α-LTX were selected after up to five panning rounds with an overall hit ratio of 15.3% and a ratio of 4.8% individual antibodies. Subsequently, 53 antibodies were subcloned and successfully produced as human IgG. The remaining 22 antibodies failed to be recloned or produced as IgG. "-" = not performed.