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. 2024 Jun 25;14:14636. doi: 10.1038/s41598-024-64592-6

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Preparation of cGLP cells of LdCen1^−/−. (a) The scheme displays the entire protocol starting from the regulatory approval till the toxicity study in the animals through the industry generated cGLP of LdCen1^−/−. (b) Diagrammatic representation of cGLP vials preparation by industrial partner. c. Growth profiles of three consecutive batches for cGLP cells counted by haemocytometer. d. Microscopic images (400X magnification) of bioreactor cultured cGLP cells on different days of growth.