Fig. 2. In vitro screening of Nt-Cys initiating peptide sequences from the human proteome with recombinant HsADO.
a Bar graphs show ADO-catalysed oxidation of 14-mer peptides representing the (Met-cleaved) Cys-initiating N-termini of potential ADO substrates (listed by gene name) after 1 min; activity was normalised to oxidation of RGS42-15 positive control peptide. Data are presented as mean ± standard deviation, n ≥ 3. b, c Sequences of peptides with which ADO was observed to have > 90% (b) or < 5% (v) activity relative to RGS42-15 positive control peptide. Amino acids colour coded according to side chain chemical properties: basic (H/K/R) = red; acidic (D/E) = blue; aromatic (F/W) = orange; polar (S/T) = purple. Source data are provided as a Source Data file.