Fig. 8. Regulation of putative ADO substrates human cells.

a, b WT, ADO KO or ADO overexpressing (OE) SH-SY5Y (a) or U87-MG (b) were exposed to hypoxia (1% O₂) for 16 h, then protein samples were collected and analysed for the expression of various potential ADO substrates. RGS4 and RGS5 were used as positive controls. c HEK 293T cells were transiently co-transfected with cDNAs encoding ANKRD29 or SUSD6, alongside either CDO1 or ADO, and subjected to hypoxia as before. d, e SH-SY5Y cells stably expressing either ANKRD29:FLAG (d) or SUSD6:FLAG (e) were subjected to hypoxia for 16 h and the expression of both proteins was analysed using an anti-FLAG antibody. RGS5 expression was also analysed as a positive control. SUSD6 was detected at a much higher level relative to RGS5 and ANKRD29 in a stably polyclonal population. All blots (a–e) are representative of at least 3 independent experiments. Source data (including relevant molecular weight markers) are provided as a Source Data file.