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. 2024 Jun 25;14:14629. doi: 10.1038/s41598-024-65092-3

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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DL predictions identify cell density changes between cortical layers. (A) Maximum intensity projection of slab 30. (B) Corresponding BCFind-v2 predictions. The highlighted region of interest (ROI) (C) without and (D) with layer contours on the raw data maximum intensity projections. The same ROI on the BCFind-v2 predictions (E) without and (F) with layer contours. Red numbers in panels D and F identify the cortical layers. Scale bars: 3 mm (A, B), 750 $μ$ m (C–F).