FIG. 3.

Endogenous RT assay. Virions were purified as described in the legend to Fig. 2B, and the endogenous RT assay was performed in the presence of 0.05% NP-40. (A) To detect the formation of −sssDNA, assays were performed for 15 min, and the products were separated on an 8% acrylamide–urea gel. (B) To monitor synthesis of longer DNA products, reactions were performed for 9 h, and the products were separated on a 1% alkaline agarose gel. The positions of migration of the −sssDNA, extended products, and DNA markers are shown on the left. As a control, 293T cells were transfected without plasmid DNA (mock).