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. 2024 May;31(5):a054013. doi: 10.1101/lm.054013.124

Figure 1.

Figure 1.

Rab3 and Nrx adult knockdown in all KCs do not alter the gross architecture of AZs. (A) Quantification of Rab3 (F(2,35) = 4.829, P = 0.0145, n = 12, post hoc Tukey's test Gal80ts;OK107/+ vs. Gal80ts;OK107/RNAi-Rab3, [*] P < 0.05) and Nrx (F(2,35) = 6.353, P = 0.0046, n = 12, post hoc Tukey's test Gal80ts;OK107/+ vs. Gal80ts;OK107/RNAi-Rab3, [**] P < 0.01) staining intensity in MB lobes of flies presenting knockdown of either Rab3 or Nrx in adult MB lobes. Representative confocal images are shown with quantitative graphics. Scale bar, 50 µm. (B) Quantification of BRPnc82 (F(2,41) = 1.704, P = 0.1953, n = 14), Syd1 (F(2,41) = 3.154, P = 0.0538, n = 14), and Unc13A (F(2,41) = 0.5814, P = 0.5639, n = 14) staining intensity in MB lobes of Gal80ts;OK107/RNAi-Rab3 flies and Gal80ts;OK107/RNAi-Nrx flies compared to control flies. Representative confocal images are shown with quantitative graphics. Scale bar, 50 µm. Data are presented as mean ± SEM. Data were analyzed using Prism (GraphPad Software). Differences were tested by one-way ANOVA with Tukey's post hoc test. Asterisks denote the least significant of the pairwise post hoc comparisons between the genotype of interest and its controls following the usual nomenclature. P > 0.05, (*) P ≤ 0.05, (**) P ≤ 0.01.